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KMID : 1094720060110040277
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Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 4 p.277 ~ p.281
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Improved immobilized enzyme systems using spherical micro silica sol-gel enzyme beads
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Lee Chang-Won
Yi Song-Se
Kim Ju-Han
Lee Yoon-Sik
Kim Byung-Gee
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Abstract
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Spherical micro silica sol-gel immobilized enzyme beads were prepared in an emulsion system using cyclohexanone and Triton-X 114. The beads were used for thein situ immobilization of transaminase, trypsin, and lipase. Immobilization during the sol to gel phase transition was investigated to determine the effect of the emulsifying solvents, surfactants, and mixing process on the formation of spherical micro sol-gel enzyme beads and their catalytic activity. The different combinations of sol-gel precursors affected both activity and the stability of the enzymes, which suggests that each enzyme has a unique preference for the silica gel matrix dependent upon the characteristics of the precursors. The resulting enzyme-entrapped micronsized beads were characterized and utilized for several enzyme reaction cycles. These results indicated improved stability compared to the conventional crushed form silica sol-gel immobilized enzyme systems.
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KEYWORD
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sol-gel immobilization, microemulsion, surfactant, Triton-X 114, cyclohexanone, transaminase, lipase, trypsin
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